Vitamin D Receptor antibody (Internal Region)

Catalog No. ABIN185419

Product Details anti-Vitamin D Receptor Antibody

Target: Vitamin D Receptor (VDR)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Vitamin D Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: VDR

Sequence: CGNQDYKYRV SD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CGNQDYKYRVSD, from the internal region of the protein sequence according to NP_000367.1, NP_001017535.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Placenta and Duodenum lysates and approx. 55-60 kDa in lysates of cell line U937 (calculated MW of 55.2 kDa according to NP_001351014.1). Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at ro

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Alternative Name: VDR (VDR Products)

Target Type: Chemical

Background: VDR, vitamin D (1,25- dihydroxyvitamin D3) receptor, HGNC:12679, NR1I1, vitamin D (1,25-dihydroxyvitamin D3) receptor

Gene ID: 7421, 22337, 24873

NCBI Accession: NP_000367, NP_001017535

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway