ADAM12 antibody (N-Term)

Catalog No. ABIN185301

Product Details anti-ADAM12 Antibody

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM12 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: ADAM12

Sequence: AARPLPVSPA RALC

Specificity: This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence AARPLPVSPARALC, from the N Terminus of the protein sequence according to NP_003465.3, NP_067673.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells. Recommended concentration: 3-5 μg/mL. Paraffin embedded Human Placenta. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ADAM12

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Alternative Name: ADAM12 (ADAM12 Products)

Background: ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha, A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of), ADAM metallopeptidase domain 12, RP11-295J3.5

Gene ID: 8038

NCBI Accession: NP_003465, NP_067673

Pathways: EGFR Signaling Pathway