BAF53A and BAF53B (C-Term) antibody
Verified
Reactivity: Human, Mouse, Rat
ELISA, WB, FACS, IF
Host: Goat
Polyclonal
unconjugated
-
- Target
- BAF53A and BAF53B
- Binding Specificity
- C-Term
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- BAF53A and BAF53B
- Sequence
- YEEGGKQCVE RKCP
- Specificity
- This antibody is expected to recognise BAF53A isoforms 1 and 2 and BAF53B, which are almost identical
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence YEEGGKQCVERKCP, from the C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1.
- Isotype
- IgG
-
- Application Notes
-
Western Blot: Approx 48 kDa band observed in Rat Skeletal Muscle lysates and approx. 50 kDa in nuclear lysates of cell line NIH3T3 and in preliminary testing of Human Colorectal cancer and Mouse Skeletal Muscle lysate.(predicted MW of 47.4 kDa (Actl6a) acco
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
-
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- BAF53A and BAF53B
- Alternative Name
- ACTL6A / ACTL6B
- Background
- BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B, 53 kDa BRG1-associated factor B, ARPN-BETA, MGC5382 Arp4, INO80K, BAF complex 53 kDa subunit, BAF53, BRG1-associated factor, INO80 com
- Gene ID
- 51412, 86
- NCBI Accession
- NP_004292, NP_817126, NP_057272
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