FARP1 antibody (N-Term)

Catalog No. ABIN185104

Product Details anti-FARP1 Antibody

Target: FARP1 (FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FARP1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: CDEP / FARP1

Sequence: GEIEQRPTPG SRL

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence GEIEQRPTPGSRL-C, from the N Terminus of the protein sequence according to NP_005757.1, NP_001001715.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments showed an approx 110-120 kDa band in Human Frontal Cortex lysates (calculated MW of 119 kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40 kDa and was successfully blocked by incuba

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FARP1

Target: FARP1 (FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1))

Alternative Name: FARP1 (FARP1 Products)

Background: FARP1, FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived), CDEP, chondrocyte-derived ezrin-like protein, RP11-111L24.1, MGC87400, PLEKH2, FERM, RhoGEF, and pleckstrin domain protein 1

Gene ID: 10160

NCBI Accession: NP_005757, NP_001001715, NP_001273768