ATP6AP2 antibody (C-Term)

Catalog No. ABIN185092

Product Details anti-ATP6AP2 Antibody

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Binding Specificity: C-Term

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP6AP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: ATP6IP2 / Renin receptor

Sequence: SIIYRMTNQK IRMD

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SIIYRMTNQKIRMD, from the C Terminus of the protein sequence according to NP_005756.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38-39 kDa band observed in Human Cerebellum and in Human and Rat Heart lysates (calculated MW of 39.0 kDa according to Human NP_005756.2 and Rat NP_001007092.1). Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour at room t

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells.. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ATP6AP2 antibody (ABIN185092)

Delforce, Lumbers, Corbisier de Meaultsart, Wang, Proietto, Otton, Scurry, Verrills, Scott, Pringle: "Expression of renin-angiotensin system (RAS) components in endometrial cancer." in: Endocrine connections, Vol. 6, Issue 1, pp. 9-19, (2017) (PubMed).

Pringle, Zakar, Yates, Mitchell, Hirst, Lumbers: "Molecular evidence of a (pro)renin/ (pro)renin receptor system in human intrauterine tissues in pregnancy and its association with PGHS-2." in: Journal of the renin-angiotensin-aldosterone system : JRAAS, Vol. 12, Issue 3, pp. 304-10, (2011) (PubMed).

Zhu, Yoneda, Demura, Karashima, Usukura, Yamagishi, Takeda: "Effect of mineralocorticoid receptor blockade on the renal renin-angiotensin system in Dahl salt-sensitive hypertensive rats." in: Journal of hypertension, Vol. 27, Issue 4, pp. 800-5, (2009) (PubMed).

Target Details for ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Alternative Name: ATP6AP2 (ATP6AP2 Products)

Background: ATP6AP2, ATP6IP2, ATPase, H+ transporting, lysosomal interacting protein 2, M8-9, APT6M8-9, ATP6M8-9, renin receptor, V-ATPase M8.9 subunit, ATPase membrane sector associated protein M8-9, vacuolar ATP synthase membrane sector associated protein M8-9, ATP

Gene ID: 10159, 70495

NCBI Accession: NP_005756

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones