PES1 antibody (N-Term)

Catalog No. ABIN185017

Product Details anti-PES1 Antibody

Target: PES1 (Pescadillo Ribosomal Biogenesis Factor 1 (PES1))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PES1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Pescadillo

Sequence: GGLEKKKYER GSA

Cross-Reactivity: Cow, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence GGLEKKKYERGSA-C, from the N Terminus of the protein sequence according to NP_055118.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows nucleolar staining in spermatocytes and spermatids. Recommended concentration, 2.5-3.8 μg/mL.

Western Blot: Approx 70 kDa band observed in HeLa lysate (calculated MW of 68 kDa according to NP_055118). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PES1

Target: PES1 (Pescadillo Ribosomal Biogenesis Factor 1 (PES1))

Alternative Name: PES1 (PES1 Products)

Background: PES1, pescadillo homolog 1, containing BRCT domain (zebrafish), PES, pescadillo (zebrafish) homolog 1, containing BRCT domain

Gene ID: 23481, 64934

NCBI Accession: NP_055118