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KAP1 antibody (C-Term)

Name: KAP1 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN184913
Price: 482.37 USD
Availability: InStock

Verified TRIM28 Reactivity: Human ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated

ABIN184913 (2µg/ml) staining of paraffin embedded Human Breast. (KAP1 antibody (C-Term))

IHC

ABIN184913 (2 µg/mL) staining of HepG2 lysate (35 µg protein in RIPA buffer). (KAP1 antibody (C-Term))

ABIN184913 (4 µg/mL) staining of paraffin embedded Human Breast. (KAP1 antibody (C-Term))

ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (KAP1 antibody (C-Term))

ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (KAP1 antibody (C-Term))

ABIN184913 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (KAP1 antibody (C-Term))

FACS

6 images

(1 reference)

Catalog No. ABIN184913

Datasheet as PDF

Target See all KAP1 (TRIM28) Antibodies

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
Binding Specificity
22

15

8

8

6

6

5

5

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
100

45

41

10

8

8

7

5

4

2

1

1

1
Human
Host
103

14

3
Goat
Compatible Secondaries
Clonality
97

23
Polyclonal
Conjugate
60

8

8

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This KAP1 antibody is un-conjugated
Application
80

36

35

27

26

22

21

21

18

13

6

4

4

3

2

2

2

1

1

1
ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose

KAP1 / TRIM28

Sequence

SSQELSGGPG DGP

Cross-Reactivity

Human, Mouse

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.

Isotype

IgG
anti-Tripartite Motif Containing 28 (TRIM28) (N-Term) antibody
TRIM28 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 Reactivity: Human WB, IF, IP, IHC (p), ICC, ChIP Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 4E6 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 576-835) antibody
TRIM28 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1D11 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 22-291) antibody
TRIM28 Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 178-207), (N-Term) antibody
TRIM28 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB09760 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (pSer824) antibody
TRIM28 Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:2000.

Comment

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).
Target

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name

TRIM28 (TRIM28 Products)

Background

TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein

Gene ID

10155

NCBI Accession

NP_005753

Pathways

Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus