NDRG1 antibody (C-Term)

Catalog No. ABIN184872

Product Details anti-NDRG1 Antibody

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NDRG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: NDRG1

Sequence: GNSAGPKSME VSC

Specificity: The variants (NP_006087.2, NP_001128714.1) encode the same protein

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence GNSAGPKSMEVSC, from the C Terminus of the protein sequence according to NP_006087.2, NP_001128714.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2μ

Western Blot: Approx 48 kDa band observed in Human Kidney, and approx 50 kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8 kDa according to NP_006087.2). This molecular weight is routinely observed by other sources. Rec

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NDRG1

Target: NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))

Alternative Name: NDRG1 (NDRG1 Products)

Background: NDRG1, N-myc downstream regulated gene 1, CAP43, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, N-myc downstream regulated gene 1 protein, differentiation-related gene 1 protein, nickel-specific induction protein Cap43, protein reg

Molecular Weight: 42.8kDa

Gene ID: 10397, 17990

NCBI Accession: NP_006087