ARPC2 antibody (C-Term)

Catalog No. ABIN184748

Product Details anti-ARPC2 Antibody

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: ARPC2

Sequence: EMKTITGKTF SSR

Specificity: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 34-35 kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3 kDa according to NP_005722.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ARPC2 antibody (ABIN184748)

Sakata, Taniguchi, Yasuda, Adachi-Morishima, Hamazaki, Nakayama, Miki, Minato, Narumiya: "Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1." in: The Journal of experimental medicine, Vol. 204, Issue 9, pp. 2031-8, (2007) (PubMed).

Target Details for ARPC2

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Alternative Name: ARPC2 (ARPC2 Products)

Background: ARPC2, actin related protein 2/3 complex, subunit 2, 34 kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2

Gene ID: 10109, 76709

NCBI Accession: NP_005722, NP_690601

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization