This Antibody recognizes c-Myc tagged fusion proteins.
Characteristics
Anti-c-Myc-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain c-Myc-tags and it is supplied as a 40 µg aliquot at a concentration of 1 mg/mL.
Purification
Immunoaffinity chromatography
Immunogen
c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 5 µg/mL Western blot: 0. 1-1. 0 µg/mL Other applications: user-optimized
Restrictions
For Research Use only
Format
Lyophilized
Concentration
0.5 mg/mL
Buffer
lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.