TRAIL antibody (N-Term)

Catalog No. ABIN184153

Product Details anti-TRAIL Antibody

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Binding Specificity: N-Term

Reactivity: Human, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAIL antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CFLKEDDSYW DPNDEESMNS PCWQVKWQLR QLVRKMILRT SEETISTVQE

Predicted Reactivity: Dog: 80%, Human: 100%, Pig: 78%

Characteristics: This is a rabbit polyclonal antibody against TNFSF10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TNFSF10

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Antigen size: 281 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TRAIL antibody (ABIN184153)

Ceballos, Parody, Quiroga, Casella, Francés, Larocca, Carnovale, Alvarez, Carrillo: "FoxO3a nuclear localization and its association with β-catenin and Smads in IFN-α-treated hepatocellular carcinoma cell lines." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 34, Issue 11, pp. 858-69, (2014) (PubMed).

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: TNFSF10 (TNFSF10 Products)

Background: TNFSF10 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
Alias Symbols: TL2, APO2L, CD253, TRAIL, Apo-2L
Protein Interaction Partner: CASP8, CASP3, CFLAR, TNFRSF10B, FADD, RIPK1, TRAF2, TNFAIP3, SOCS3, RBM48, TNFRSF10D, TNFRSF10C, TNFRSF10A, TNFSF10, TNFRSF11B, ACP5, IER3, CREBBP, SP3, SP1, HDAC5, HDAC4, HDAC3, HDAC2, HDAC1, HIST3H3,
Protein Size: 281

Molecular Weight: 33 kDa

Gene ID: 8743

NCBI Accession: NM_003810, NP_003801

UniProt: P50591

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity