ZNF496 antibody (N-Term)

Catalog No. ABIN183828

Product Details anti-ZNF496 Antibody

Target: ZNF496 (Zinc Finger Protein 496 (ZNF496))

Binding Specificity: N-Term

Reactivity: Mouse, Human, Rat, Cow, Sheep, Dog, Horse, Pig, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF496 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MPTALCPRVL APKESEEPRK MRSPPGENPS PQGELPSPES SRRLFRRFRY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against ZNF496. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZNF496

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 587 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ZNF496

Target: ZNF496 (Zinc Finger Protein 496 (ZNF496))

Alternative Name: ZNF496 (ZNF496 Products)

Background: ZNF496 contains a SCAN box, a KRAB-A domain, and four consensus C2H2-type zinc fingers preceded by a unique finger derivative, referred to herein as the C2HR motif. The C2HR motif functions to mediate protein-protein interaction with the cysteine-rich (C5HCH) domain of NSD1 in a Zn(II)-dependent fashion, and when tethered to RNA polymerase II promoters, represses transcription in an NSD1-dependent manner. ZNF496 contains a novel type of zinc finger motif that functions as a docking site for NSD1 and is more than just a degenerate evolutionary remnant of a C2H2 motif.
Alias Symbols: NIZP1, ZFP496, ZKSCAN17
Protein Interaction Partner: ZKSCAN4, ZSCAN22, ZNF483, ZNF446, ZSCAN12, ZSCAN21, ARR3, Nsd1, SUMO2, UBC, CBX3, TRIM28, NR3C1, POLR3D, ZZZ3, JADE2, RAI1, SSX3, SUPT5H, PPARG,
Protein Size: 587

Molecular Weight: 67 kDa

Gene ID: 84838

NCBI Accession: NM_032752, NP_116141

UniProt: Q96IT1