SPI1 antibody (N-Term)

Catalog No. ABIN183577

Product Details anti-SPI1 Antibody

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: NEFENFPENH FTELQSVQPP QLQQLYRHME LEQMHVLDTP MVPPHTGLSH

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 79%, Mouse: 100%, Rabbit: 86%, Rat: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SFPI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of mouse SFPI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 272 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SFPI1 (SPI1 Products)

Background: Sfpi1 binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Sfpi1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Sfpi1 also binds RNA and may modulate pre-mRNA splicing.
Alias Symbols: Dis1, PU.1, Spi1, Dis-1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1, SFPI1
Protein Interaction Partner: Prdm1, Jun, Hoxa10, Ep300, Kat6a, Irf4, Pml, Trim33, Cbfa2t3, Sin3a, Hdac2, Id2, Gata1, Emx1, Crebbp, Gata3, Csf1r, Il7r,
Protein Size: 272

Molecular Weight: 30 kDa

Gene ID: 20375

NCBI Accession: NM_011355, NP_035485

UniProt: P17433

Pathways: Stem Cell Maintenance