PYCARD antibody (Middle Region)

Catalog No. ABIN183475

Product Details anti-PYCARD Antibody

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Binding Specificity: Middle Region

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TVLRDMGLQE LAEQLQTTKE ESGAVAAAAS VPAQSTARTG HFVDQHRQAL

Predicted Reactivity: Mouse: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against PYCARD. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse PYCARD

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 193 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: PYCARD (PYCARD Products)

Background: Pycard is a cytosolic soluble protein that forms insoluble aggregates and enhances etoposide-induced apoptosis.
Alias Symbols: Asc, TNS1, masc, CARD5, TMS-1, 9130417A21Rik
Protein Size: 193

Molecular Weight: 21 kDa

Gene ID: 66824

NCBI Accession: NM_023258, NP_075747

UniProt: Q9EPB4

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome