ATF4 antibody (N-Term)

Catalog No. ABIN183436

Product Details anti-ATF4 Antibody

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MALFTKSSSS VAVTDKDTFE LSTFLESSKA PQHDRDELPE QRSVGGGLDD

Predicted Reactivity: Mouse: 100%

Characteristics: This is a rabbit polyclonal antibody against ATF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of mouse ATF4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Antigen size: 381 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATF4 antibody (ABIN183436)

Ye, Zhang, Ancrum, Manevich, Townsend, Tew: "Glutathione S-Transferase P-Mediated Protein S-Glutathionylation of Resident Endoplasmic Reticulum Proteins Influences Sensitivity to Drug-Induced Unfolded Protein Response." in: Antioxidants & redox signaling, (2016) (PubMed).

Schneeberger, Dietrich, Sebastián, Imbernón, Castaño, Garcia, Esteban, Gonzalez-Franquesa, Rodríguez, Bortolozzi, Garcia-Roves, Gomis, Nogueiras, Horvath, Zorzano, Claret: "Mitofusin 2 in POMC neurons connects ER stress with leptin resistance and energy imbalance." in: Cell, Vol. 155, Issue 1, pp. 172-87, (2013) (PubMed).

Drivas, Holzbaur, Bennett: "Disruption of CEP290 microtubule/membrane-binding domains causes retinal degeneration." in: The Journal of clinical investigation, Vol. 123, Issue 10, pp. 4525-39, (2013) (PubMed).

Alger, Rayavarapu, Nagaraju: "Measurement of activation of the endoplasmic reticulum stress response in autoimmune myositis." in: Methods in enzymology, Vol. 489, pp. 207-25, (2011) (PubMed).

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4 (ATF4 Products)

Background: Atf4 binds to asymmetric cAMP response elements (CRE) as a heterodimer and to palindromic CRE's as a homodimer.
Alias Symbols: Atf-4, C/ATF, CREB2, TAXREB67
Protein Interaction Partner: Egln3, Ubc, Hif1a, Satb2, Fam175b, Trib3, Gabbr2, Gabbr1, Jun, Fos, Dapk3, Rps6ka3, Tnfsf11, Prkaca,
Protein Size: 381

Molecular Weight: 42 kDa

Gene ID: 11911

NCBI Accession: NM_009716, NP_033846

UniProt: Q61328

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response