APEX1 antibody (N-Term)

Catalog No. ABIN183434

Product Details anti-APEX1 Antibody

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Binding Specificity: N-Term

Reactivity: Mouse, Rat, Cow, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MPKRGKKAAA DDGEEPKSEP ETKKSKGAAK KTEKEAAGEG PVLYEDPPDQ

Predicted Reactivity: Cow: 85%, Guinea Pig: 86%, Horse: 79%, Mouse: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against APEX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 317 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1 (APEX1 Products)

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells.
Alias Symbols: APE, Apex, HAP1, Ref-1
Protein Interaction Partner: Pcna, Gadd45a, Eed, Mutyh, Plcb1, Hdac1, Sin3a, Hdac2,
Protein Size: 317

Molecular Weight: 35 kDa

Gene ID: 11792

NCBI Accession: NM_009687, NP_033817

UniProt: Q544Z7

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus