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CD19 antibody (AA 422-433)

CD19 Reactivity: Human WB, ELISA Host: Goat Polyclonal unconjugated

Catalog No. ABIN1833920

Datasheet as PDF

Target See all CD19 Antibodies

CD19 (CD19 Molecule (CD19))
Binding Specificity
23

16

16

14

10

7

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 422-433
Reactivity
281

148

41

29

19

10

7

5

4

3

2

2

2

2

2

2

2

2
Human
Host
171

130

41

4
Goat
Compatible Secondaries
Clonality
227

115

2
Polyclonal
Conjugate
122

22

21

20

17

8

8

7

7

7

5

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD19 antibody is un-conjugated
Application
253

113

70

67

51

49

23

21

14

14

9

8

5

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA

Specificity

Human CD19. This antibody is expected to recognize both reported isoforms (NP_001171569.1, NP_001761.3).

Purification

Immunoaffinity purified

Immunogen

Synthetic peptide C-ENDSNLGQDQLS from an internal region of human CD19 (NP_001171569.1, NP_001761.3). Percent identity by BLAST analysis: Hamster, Panda, Pig, Guinea pig (92%), Gibbon, Ferret, Rabbit (83%).

Type of Immunogen: Synthetic peptide

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Application Notes

Approved: Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kD according to NP_NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 μg/mL.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C. Avoid freeze-thaw cycles.
Target

CD19 (CD19 Molecule (CD19))

Alternative Name

CD19 (CD19 Products)

Background

Name/Gene ID: CD19

Synonyms: CD19, B-lymphocyte antigen CD19, CD19 Molecule, Differentiation antigen CD19, T-cell surface antigen Leu-12, B4, CD19 antigen, CVID3

Gene ID

930

NCBI Accession

NP_001171569, NP_001761

UniProt

P15391

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway