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Histone 3 antibody (H3K9me3)

H3 Reactivity: Human, Rat, Monkey WB, IF, IP Host: Mouse Monoclonal B1249M unconjugated

Catalog No. ABIN1822548

Datasheet as PDF

Target See all Histone 3 (H3) Antibodies

Histone 3 (H3)
Binding Specificity
57

49

47

42

40

36

36

35

34

34

34

33

32

30

27

27

26

23

23

22

22

18

17

17

16

16

16

16

15

15

15

14

13

13

13

13

12

12

12

11

11

10

10

10

10

9

9

8

8

8
H3K9me3
Reactivity
1355

928

854

58

41

41

40

40

39

35

34

30

26

22

19

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Human, Rat, Monkey
Host
1175

238

8

5
Mouse
Compatible Secondaries
Clonality
1098

327

1
Monoclonal
Conjugate
794

77

44

44

44

44

44

44

34

33

21

18

18

18

17

17

17

17

17

17

17

17

12

1
This Histone 3 antibody is un-conjugated
Application
1032

406

374

314

240

188

172

166

163

155

139

128

116

48

36

34

32

22

16

14

7

7

6

4

3

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity

Endogenous levels of histone H3 when di- or tri-methylated on Lys9. Species Crossreactivity: human, monkey, rat.

Purification

Purified

Immunogen

Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is tri-methylated.

Type of Immunogen: Synthetic peptide

Clone

B1249M

Isotype

IgG1

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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: IF (1:100), IP (1:100), WB

Usage: Western Blot: 1:2000 Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, less than 0.02 % sodium azide, 50 % glycerol

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

4 °C,-20 °C

Storage Comment

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
Target

Histone 3 (H3)

Alternative Name

Histone H3 (H3 Products)

Synonyms

H-3 antibody, histocompatibility 3 antibody, H3 antibody