CD58 antibody (FITC)

Catalog No. ABIN1821093

Product Details anti-CD58 Antibody (FITC)

Target: CD58

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD58 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Human CD58 glycoprotein of about 60-70kD. Inhibits HLA-DR mediated T cell cytotoxicity

Purification: Protein A purified

Immunogen: Human cytolytic T cells.

Type of Immunogen: Cells

Clone: TS2-9-1-4-3

Isotype: IgG1

Application Details

Application Notes: Approved: Flo

Usage: Flow Cytometry: 5x10e5 cultured Nalm-6 cells were washed after which they were incubated 45 min on ice with 80 μL of anti-CD58 antibody at 10 μg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.46 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing. Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for CD58

Target: CD58

Alternative Name: CD58 (CD58 Products)

Background: Name/Gene ID: CD58
Family: Immunoglobulin

Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule

Gene ID: 965

UniProt: P19256