CD45 antibody (FITC)

Catalog No. ABIN182034

Product Details anti-CD45 Antibody (FITC)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Dog

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: CD45R

Purification: Affinity chromatography on Protein G

Immunogen: Canine thymocytes. Spleen cells from immunised DA rat were fused with cells of the Y3/A1.2.3 rat myeloma cell line

Clone: YKIX753-22-2

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Neat - 1/5, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 (PTPRC Products)

Synonyms: PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

Background: CD45 is expressed on all leukocytes in canine peripheral blood and may be against the CD45R isoforms. CD45R is expressed on subsets of leukocytes (neutrophils are weak) in canine blood of all out bred mongrels and beagles tested.Synonyms: CD45R, L-CA, Leukocyte common antigen, PTPRC, T200

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling