alpha Tubulin antibody (Subunit alpha) (HRP)

Catalog No. ABIN1820258

Product Details anti-alpha Tubulin Antibody (HRP)

Target: alpha Tubulin (TUBA1)

Binding Specificity: Subunit alpha

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Arabidopsis, Drosophila melanogaster, Xenopus laevis, C. elegans

Host: Rat

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

No Cross-Reactivity: Nephrotoma

Purification: Protein G purified

Immunogen: Yeast tubulin.

Type of Immunogen: Purified protein

Clone: YL1-2

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, WB (1:100 - 1:1000)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Yeast

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % Thimerosal, HRP stabilizer

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin (TUBA1 Products)

Synonyms: Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody

Pathways: Microtubule Dynamics