DLL4 antibody

Catalog No. ABIN1820181

Product Details anti-DLL4 Antibody

Target: DLL4 (delta-Like 4 (DLL4))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This DLL4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumors, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting non-productive angiogenesis. Clone HMD4-2 has been shown to block mouse Notch1-Fc and Notch4-Fc binding to mouse DLL4/CHO cells, and to inhibit subcutaneous tumor growth in mouse tumor models using the murine lung carcinoma cell line KLN205. Removal of Sodium Azide is recommended prior to use in functional assays.

Purification: Protein G purified

Immunogen: Recombinant mouse DLL4.

Type of Immunogen: Recombinant protein

Clone: HMD4-2

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Alternative Name: DLL4 (DLL4 Products)

Background: Name/Gene ID: DLL4

Synonyms: DLL4, Delta-like 4 protein, Delta like 4, Delta-like 4 (Drosophila), Delta-like 4 homolog, Drosophila Delta homolog 4, Notch ligand DLL4, Delta 4, Delta ligand 4, Delta-like protein 4, Delta4, Hdelta2, Notch ligand delta-2

Gene ID: 54567

UniProt: Q9NR61

Pathways: Notch Signaling