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CD3 antibody (FITC)

CD3 Reactivity: Human FACS Host: Rat Monoclonal CD3-12 FITC

Catalog No. ABIN1819664

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
177

98

24

20

17

10

8

8

7

7

5

3

2

1
Human
Host
210

47

20

13

9

1
Rat
Compatible Secondaries
Clonality
289

6

1
Monoclonal
Conjugate
93

39

32

23

23

11

8

6

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is conjugated to FITC
Application
274

81

58

52

45

38

32

22

9

8

7

6

4

3

2

2

2

2

2

2

2

2

1

1
Flow Cytometry (FACS)

Specificity

Raised against a peptide representing an invariant cytoplasmic sequence within the CD3epsilon chain recognizes human CD3epsilon. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilon-gamma, epsilon-delta, zeta-zeta,). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) , ,. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. Ashas been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross-reactivity for the CD3 marker. ,.

Purification

Protein G purified

Immunogen

Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys). Percent identity by BLAST analysis: Chimpanzee, Gorilla, Ferret, Panda, Bovine, Dog, Cat, Opossum, Turkey, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Clone

CD3-12

Isotype

IgG1

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Application Notes

Approved: Flo (1:5 - 1:10)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.09 % sodium azide, 1 % BSA

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing. Protect from light.

Storage

4 °C,-20 °C

Storage Comment

Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Target

CD3

Alternative Name

CD3 (CD3 Products)

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway