MHC Class II DR antibody (FITC)
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- Target
- MHC Class II DR
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Reactivity
- Sheep
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS)
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Splenocytes from ATL mice.
- Clone
- 37-68
- Isotype
- IgG2a
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- Application Notes
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Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- MHC Class II DR
- Background
- The distribution of ovine DR molecules on T lymphocytes has been shown to vary with immune status and age. Initially T lymphocytes lack class II molecules, then T lymphocytes progressively express DR molecules.
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