V5 Epitope Tag antibody
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- Target See all V5 Epitope Tag products
- V5 Epitope Tag
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This V5 Epitope Tag antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
- Sequence
- GKPIPNPLLG LDST
- Purification
- Affinity chromatography on Protein G
- Immunogen
- Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
- Clone
- SV5-PK1
- Isotype
- IgG2a
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- Application Notes
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Immunoprecipitation. Radioimmunoassasy. Immunohistochemistry on frozen sections. ELISA: 1/1000 - 1/5000. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- V5 Epitope Tag
- Alternative Name
- Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag Products)
- Target Type
- Tag
- Background
- The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
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