CD68 antibody

Catalog No. ABIN181837

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This monoclonal FA-11 antibody does detect surface Macrosialin at low levels in resident Mouse peritoneal Macrophages which can be enhanced with thioglycollate stimulation.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G

Immunogen: Purified Concanavalin A acceptor glycoprotein from P815 cell line.

Clone: FA-11

Isotype: IgG2a

Application Details

Application Notes: Western Blot. Immunoprecipiation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD68 antibody (ABIN181837)

Asdonk, Steinmetz, Krogmann, Ströcker, Lahrmann, Motz, Paul-Krahe, Flender, Schmitz, Barchet, Hartmann, Nickenig, Zimmer: "MDA-5 activation by cytoplasmic double-stranded RNA impairs endothelial function and aggravates atherosclerosis." in: Journal of cellular and molecular medicine, Vol. 20, Issue 9, pp. 1696-705, (2017) (PubMed).

Jehle, Hoyer, Schöne, Pfeifer, Schild, Jenniches, Bindila, Lutz, Lütjohann, Zimmer, Nickenig: "Myeloid-Specific Deletion of Diacylglycerol Lipase α Inhibits Atherogenesis in ApoE-Deficient Mice." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146267, (2016) (PubMed).

Su, Lu, Kenens, Rocha, Fron, Yuan, Chen, Van Dorpe, Roeffaers, Mizuno, Hofkens, Hutchison, Uji-I: "Visualization of molecular fluorescence point spread functions via remote excitation switching fluorescence microscopy." in: Nature communications, Vol. 6, pp. 6287, (2015) (PubMed).

Papadaki, Politou, Smirlis, Kotini, Kourou, Papamarcaki, Boleti: "The Leishmania donovani histidine acid ecto-phosphatase LdMAcP: insight into its structure and function." in: The Biochemical journal, Vol. 467, Issue 3, pp. 473-86, (2015) (PubMed).

Zimmermann, Homann, Bangert, Müller, Hristov, Goeser, Wiehe, Zittrich, Rottbauer, Torzewski, Pfitzer, Katus, Kaya: "Successful use of mRNA-nucleofection for overexpression of interleukin-10 in murine monocytes/macrophages for anti-inflammatory therapy in a murine model of autoimmune myocarditis." in: Journal of the American Heart Association, Vol. 1, Issue 6, pp. e003293, (2013) (PubMed).

Konwisorz, Springwald, Haselberger, Goerse, Ortmann, Treeck: "Knockdown of ICB-1 gene enhanced estrogen responsiveness of ovarian and breast cancer cells." in: Endocrine-related cancer, Vol. 17, Issue 1, pp. 147-57, (2010) (PubMed).

Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).

Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).

Casati, Camisaschi, Novellino, Mazzocchi, Triebel, Rivoltini, Parmiani, Castelli: "Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3782-8, (2008) (PubMed).

Ryu, Lee, Hong, Shin, Lim, Park, Shim, Nam, Choi, Kim, Han: "Activation of fractalkine/CX3CR1 by vascular endothelial cells induces angiogenesis through VEGF-A/KDR and reverses hindlimb ischaemia." in: Cardiovascular research, Vol. 78, Issue 2, pp. 333-40, (2008) (PubMed).

Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin

Gene ID: 12514

NCBI Accession: NP_033983

UniProt: P31996