CD68 antibody (FITC)

Catalog No. ABIN181834

Product Details anti-CD68 Antibody (FITC)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD68 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The antibody recognizes CD68. SM1550F does detect surface macrosialin at low levels in resident peritoneal macrophages which can be enhanced with thioglycollate stimulation.

Purification: Affinity Chromatography on Protein G.

Clone: FA-11

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: use 10 μL of neat - 1:10 diluted antibody to label 10^6 cells in 100 μL. Membrane permeabilisation is required for this application. ABIN181834 does detect surface macrosialin at low levels in resident peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD68 antibody (FITC) (ABIN181834)

Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin

Gene ID: 12514

NCBI Accession: NP_033983

UniProt: P31996