TGFB1 antibody

Catalog No. ABIN181152

Product Details anti-TGFB1 Antibody

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects TGF-3.

Characteristics: Synonyms: Transforming growth factor beta-1, TGFB, LAP, Latency-associated peptide

Purification: Affinity Chromatography on Protein G

Immunogen: Highly pure (>98%) recombinant Human TGFB1

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human TGFB1 when used inconjunction with compatible secondary reagents. Western Blot: To detect Human TGFB1 by Western Blot analysis this antibody can be usedat a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human TGFB1 is 2.0-4.0 ng/lane, undernon-reducing conditions. In a dot blot, a 1/500 dilution of this antibody exhibited complete cross reactivity withHuman TGFB2 and TGFB3 isotypes. Reduced cross reactivity was shown at a 1/1000dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1 (TGFB1 Products)

Background: Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1

Gene ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints