Complement C4A/C4B antibody

Catalog No. ABIN181130

Product Details

Target: Complement C4A/C4B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Specificity: This antibody recognises a neoantigen on the secreted protein complement component 4d (C4d).

Characteristics: Synonyms: Complement component 4, CPAMD2, CPAMD3, Acidic complement C4, Basic complementC4

Purification: Affinity chromatography on Protein A

Immunogen: Purified human C4d. Genename: C4d

Clone: 057-51-5-1-6

Isotype: IgG1

Application Details

Application Notes: ELISA. Flow Cytometry. Immunohistochemistry on frozen sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Borate buffered saline pH 8.2 - 8.4 containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Complement C4A/C4B

Synonyms: C4 antibody, C4-1 antibody, C4b antibody, complement component 4A (Rodgers blood group) antibody, C4a antibody

Background: The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. The short half life of C4d means that it is present in serum at high enough concentrations to make it a useful marker for classical complement activation. C4 has been linked to susceptibility to systemic lupus erythematosous.Synonyms: Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4

Gene ID: 720

UniProt: P0C0L4