C5 antibody

Catalog No. ABIN181110

Product Details anti-C5 Antibody

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody recognizes secreted complement component 5 (C5), a 190 kDa serine protease present in blood serum. In haemolysis inhibition studies it was found that this antibody will block C5 haemolytic activity. Removal of Sodium Azide is recommended prior to use in Functional Assays.

Characteristics: Synonyms: CPAMD4, Complement component 5, C3 and PZP-like alpha-2-macroglobulindomain-containing protein 4

Purification: Affinity Chromatography on Protein A

Immunogen: Purified human C5Genename: C5

Clone: 10-04-14

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Borate buffered saline, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: Complement C5 (C5 Products)

Background: It is composed of an 115 kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5

Gene ID: 727

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis