EBV antibody

Catalog No. ABIN181081

Product Details anti-EBV Antibody

Target: EBV (Epstein-Barr Virus (EBV))

Reactivity: Epstein-Barr Virus (EBV)

Host: Rat

Clonality: Monoclonal

Conjugate: This EBV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: Synonyms: HHV4, Epstein Barr Virus LMP2A

Purification: Affinity Chromatography on Protein G

Immunogen: Bacterial TrpE-LMP2A fusion protein. Spleen cells from immunised Lou/c rats were fusedwith cells of the P3X63. Ag8. 653 myeloma cell line.

Clone: 15F9

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1/100 - 1/1000. Immunohistochemistry on paraffin sections: This product requires antigen retrieval usingheat treatment methods prior to staining of paraffin sections. Sodium citrate buffer pH 6.0is recommended for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for EBV

Target: EBV (Epstein-Barr Virus (EBV))

Alternative Name: Epstein Barr Virus / EBV (EBV Products)

Target Type: Virus

Background: EBV is a human herpesvirus, which is associated with conditions such as Hodgkins disease and Burkitts Lymphoma and is the causative agent in mononucleosis in adolescents. EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases. Studies show that LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals.Synonyms: HHV-4, HHV4