NGFR antibody (N-Term)

Catalog No. ABIN180341

Product Details anti-NGFR Antibody

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Binding Specificity: AA 1-160, N-Term

Reactivity: Human, Rabbit, Cat, Ferret, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: This antibody recognizes CD271/NGFR, a transmembrane glycoprotein of the TNFR superfamily. The epitope is localized within amino acids 1-160.
Cellular Localization: Cell Surface, Cytoplasmic.

Cross-Reactivity (Details): Species reactivity (tested):Human, Monkey (Cebus apella, Macaca fascicularis, mulatta, and nemestrina), Baboon (Papio anubis and Cynocephalus), Cat, Rabbit, and Ferret.

Characteristics: Synonyms: TNFRSF16, Tumor necrosis factor receptor superfamily member 16

Purification: Protein-A affinity chromatography

Immunogen: NGFR (75kD) from A875 Melanoma Cells.

Clone: NGFR5

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunocytochemistry. Inhibits Binding of NGF to Melanoma Cells (see Ref.1, use antibody without azide: ABIN180340). Not suitable for Western Blotting andImmunohistochemistry. Recommended Positive Control: Melanoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer. Avoid repeated freezethaw cycles!
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-NGFR antibody (ABIN180341)

Molina-Ruiz, Cerroni, Kutzner, Requena: "Immunohistochemistry in the diagnosis of cutaneous bacterial infections." in: The American Journal of dermatopathology, Vol. 37, Issue 3, pp. 179-93; quiz 194-6, (2015) (PubMed).

Alpers, Hudkins, Ferguson, Johnson, Schatteman, Bothwell: "Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 703-13, (1994) (PubMed).

Schatteman, Gibbs, Lanahan, Claude, Bothwell: "Expression of NGF receptor in the developing and adult primate central nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 3, pp. 860-73, (1988) (PubMed).

Marano, Dietzschold, Earley, Schatteman, Thompson, Grob, Ross, Bothwell, Atkinson, Koprowski: "Purification and amino terminal sequencing of human melanoma nerve growth factor receptor." in: Journal of neurochemistry, Vol. 48, Issue 1, pp. 225-32, (1987) (PubMed).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: CD271 / NGFR (NGFR Products)

Background: CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.Synonyms: TNFRSF16, Tumor necrosis factor receptor superfamily member 16

Molecular Weight: 75 kDa

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding