ARPC5 antibody (AA 19-24)

Catalog No. ABIN1742556

Product Details anti-ARPC5 Antibody

Target: ARPC5 (Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5))

Binding Specificity: AA 19-24

Reactivity: Human, Rat, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARPC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Specific for p16-Arc.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant full length p16-Arc. Epitope YDENKF (aa 19-24 in human p16-Arc).

Clone: 323H3

Isotype: IgG2a

Application Details

Application Notes: WB: 1 : 100 up to 1 : 2000 (AP staining)
IP: not tested yet
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-ARPC5 antibody (ABIN1742556)

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, (2011) (PubMed).

Marion, Hoffmann, Holzer, Le Clainche, Martin, Sachse, Ganeva, Mangeat, Griffiths: "Ezrin promotes actin assembly at the phagosome membrane and regulates phago-lysosomal fusion." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 4, pp. 421-37, (2011) (PubMed).

Lladó, Timpson, Vilà de Muga, Moretó, Pol, Grewal, Daly, Enrich, Tebar: "Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin." in: Molecular biology of the cell, Vol. 19, Issue 1, pp. 17-29, (2008) (PubMed).

Steffen, Faix, Resch, Linkner, Wehland, Small, Rottner, Stradal: "Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes." in: Molecular biology of the cell, Vol. 17, Issue 6, pp. 2581-91, (2006) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for ARPC5

Target: ARPC5 (Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5))

Alternative Name: p16-Arc (ARPC5 Products)

Background: Synonyms: Arp C5

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization