ARPC3 antibody (AA 109-120)

Catalog No. ABIN1742555

Product Details anti-ARPC3 Antibody

Target: ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))

Binding Specificity: AA 109-120

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARPC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: Specific for p21-Arc.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant full length p21-Arc. Epitope PGFPLNAIYAKP (aa 109-120 in human p21-Arc).

Clone: 24A6

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 100 up to 1 : 500 (AP staining)
IP: not tested yet
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-ARPC3 antibody (ABIN1742555)

Rodriguez-Espinosa, Fernandez-Espejo: "Effects of acute and repeated cocaine on markers for neural plasticity within the mesolimbic system in rats." in: Psychopharmacology, Vol. 232, Issue 1, pp. 57-62, (2015) (PubMed).

Gonzalez-Aparicio, Flores, Fernandez-Espejo: "Antiparkinsonian trophic action of glial cell line-derived neurotrophic factor and transforming growth factor β1 is enhanced after co-infusion in rats." in: Experimental neurology, Vol. 226, Issue 1, pp. 136-47, (2010) (PubMed).

Steffen, Faix, Resch, Linkner, Wehland, Small, Rottner, Stradal: "Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes." in: Molecular biology of the cell, Vol. 17, Issue 6, pp. 2581-91, (2006) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for ARPC3

Target: ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))

Alternative Name: p21-Arc (ARPC3 Products)

Background: Synonyms: Arp C3

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization