GFP antibody (CypHer5E)
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- Target See all GFP Antibodies
- GFP (Green Fluorescent Protein (GFP))
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Reactivity
- Aequorea victoria
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GFP antibody is conjugated to CypHer5E
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Application
- Immunocytochemistry (ICC)
- Cross-Reactivity (Details)
- Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).
- Purification
- Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization.
- Immunogen
- Strep-Tag® fusion protein, full-length GFP.
- Top Product
- Discover our top product GFP Primary Antibody
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- Application Notes
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IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet - Comment
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ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
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Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.
Prolonged storage at 4 °C is not recommended!
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How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
: "
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How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
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- Target
- GFP (Green Fluorescent Protein (GFP))
- Alternative Name
- GFP (GFP Products)
- Synonyms
- green fluorescent protein antibody, gfp antibody
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