SIGLEC12 antibody (AA 27-141)

Catalog No. ABIN1174413

Product Details anti-SIGLEC12 Antibody

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Binding Specificity: AA 27-141

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12)

Specificity: The antibody is a rabbit polyclonal antibody raised against SIGLEC12. It has been selected for its ability to recognize SIGLEC12 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12) corresdonding to Leu27~Met141 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.2-2 μg/mL

1:250-2500 Immunohistochemistry: 5-20 μg/mL

1:25-100 Immunocytochemistry: 5-20 μg/mL

1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SIGLEC12

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Alternative Name: Sialic Acid Binding Ig Like Lectin 12 (SIGLEC12 Products)

Background: S2V, SIGLECL1, SLG, Siglec-L1, Siglec-XII, Sialic acid-binding Ig-like lectin-like 1