WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Restrictions
For Research Use only
Concentration
1mg/mL
Buffer
10mM PBS, pH 7.2, 0.05% NaN3
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Chen, Wang, Garate, Zhou, Li: "The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system." in: Oncogene, Vol. 29, Issue 10, pp. 1498-508, (2010) (PubMed).