ELTD1 antibody (AA 301-400)

Catalog No. ABIN1713780

Product Details anti-ELTD1 Antibody

Target: ELTD1 (EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1))

Binding Specificity: AA 301-400

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELTD1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ETL/ELTD1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Independent Validation (IHC (fp))

Validation #100001 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100001

Date: 10/15/2015

Antigen: ELTD1

Method validated: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Positive Control: Tumor cell transplanted mouse brain was dissected

Notes: The staining pattern was as expected for ELTD1 as membrane protein.

Validation Images

IF validation of ELTD1 antibody ABIN1713780 on mouse brain tumor sections. A. ABIN1713780 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.

Full Methods

Primary Antibody: ABIN1713780

Secondary Antibody: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• Dry slides at 50-55°C for 15-30 min (or RT for 30min)
• Wash three times with PBS 5min (shake gently)
• Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
• Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
• Rabbit-anti-ELTD1 antibody (ABIN1713780) diluted 1:50-1:400
• Wash three times with PBS 5min (shake gently)
• Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
• Donkey-anti-Rabbit-Cy3
• DAPI 1:100
• Wash three times with PBS 5min (shake gently)
• Add three drops of water-based Immu-mount
• Cover with 24x60 cover glass
• Imaging
• Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Experimental Notes: ABIN1713780 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ELTD1

Target: ELTD1 (EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1))

Alternative Name: ETL/ELTD1 (ELTD1 Products)

Background:

Synonyms: ETL, ELTD1, KPG_003, Adhesion G protein-coupled receptor L4, EGF, latrophilin and seven transmembrane domain-containing protein 1, EGF-TM7-latrophilin-related protein, ETL protein, ADGRL4, UNQ202/PRO228

Background: Endothelial orphan receptor that acts as a key regulator of angiogenesis.

Gene ID: 64123

UniProt: Q9HBW9