CD56 antibody
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- Target See all CD56 (NCAM1) Antibodies
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD56 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody reacts with a 135/220 kD glycosylated and sialyated protein, expressed on CD2+, CD3-, CD16+ natural killer cells and neuroectodermal cells.
- Purification
- Purified.
- Immunogen
- Membrane preparation from small cell lung carcinoma.
- Clone
- B332(123A8)
- Isotype
- IgG1
- Top Product
- Discover our top product NCAM1 Primary Antibody
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- Application Notes
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Immunofluorescence: 10-20 micrograms/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Westernblotting: 1-5 ug/mL.
Immunohistochemistry: 4 -5 micrograms/mL on frozen and formalin fixed tissue sections using avidin-biotin system. The formalin fixed tissue requires boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. This antibody is used for identification of NK- cells.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections. This antibody can be used with formalin-fixed paraffin-embedded tissue sections. These tissue require boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling at RT. The antibody may be used at a dilution of 2-4 micrograms/mL in IHC. - Comment
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Recommended Positive Control: Human retinoblastoma or neuroblastoma, small Granulocytes Lung Carcinoma or wilms tumors.
- Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/ml.
- Buffer
- 10 mM Sodium phosphate, 150 mM Sodium Chloride, 0.2% BSA, pH 7.4
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
- Alternative Name
- CD56,Natural Killer Cell (NCAM1 Products)
- Molecular Weight
- 125, 145, 180 kDa.
- Gene ID
- 4684
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