BEC12 antibody
Reactivity: Mouse
FACS, IHC, WB
Host: Rat
Monoclonal
LA5
unconjugated
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- Target
- BEC12
- Reactivity
- Mouse
- Host
- Rat
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- With collagenase- and neuraminidase-treated mouse benign lymphangiomas
- Characteristics
- The antibody was produced by a rapid differential immunization of DA rats (licenced from Tokyo Women’s Medical University, Japan) with collagenase- and neuraminidase-treated mouse benign lymphangiomas.
- Purification
- The rat IgG2a antibody was purified from cell culture supernatant by Protein G chromatography.
- Clone
- LA5
- Isotype
- IgG2a
-
- Comment
-
Monoclonal Antibody
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS
- Handling Advice
- Centrifuge vial prior to opening.
- Storage
- -20 °C
- Storage Comment
- The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.
- Expiry Date
- 6 months
-
- Target
- BEC12
- Background
-
LA5 reacts with most mouse blood vessels except heart, large arteries and veins, but not with any lymphatic vessels. LA5 recognized a protein of around 12 kDa. The antigen recognized by LA5 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides blood vessels, some lymphoid cells are also recognized.
Synonyms: Blood endothelial cell marker 12
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