For best experience we recommend to activate Javascript in your browser.

GFP antibody

GFP Reactivity: Aequorea victoria WB Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN1589916

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
175

15

11

9
Aequorea victoria
Host
64

63

34

13

9

5

2

1
Rabbit
Compatible Secondaries
Clonality
113

73
Polyclonal
Conjugate
82

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
148

80

47

34

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB)

Purpose

GFP antibody

Characteristics

The anti-GFP antibody was generated by immunization of rabbits with highly purified recombinant GFP protein produced in E. coli.

Purification

The anti-GFP antibodies were purified by affinity chromatography with immobilized Protein A.

Isotype

IgG

Top Product

Discover our top product GFP Primary Antibody
anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Goat Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea aequorea WB, IF, IHC (fro), IHC (p) Host: Goat Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, IF, IP, FACS Host: Mouse Monoclonal 6C11C11 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria WB, ELISA Host: Mouse Monoclonal 9F9-F9 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq Host: Rat Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, IHC, FACS Host: Mouse Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Rabbit Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Western Blot: Use 2-5 μg/mL

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer

PBS

Handling Advice

Centrifuge vial prior to opening.

Storage

4 °C,-20 °C

Storage Comment

The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date

24 months
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Green fluorescent protein,Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues. The antibody was made against GFP from the jellyfish Aequorea Victoria using the whole native protein as an antigen.