CEACAM1 antibody

Catalog No. ABIN1589708

Product Details anti-CEACAM1 Antibody

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Purpose: CEACAM-1 antibody

Specificity: Recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)

Cross-Reactivity (Details): Anti-human CEACAM-1 (B3) interacts solely with human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in human or other species!

Characteristics: Chromosomal location: 19q13.2
Monoclonal antibodies were produced with the help of BALB/c mice using recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells) as the immunizing antigen.

Purification: The antibody (clone #B3) was purified from cell culture supernatant by Protein G chromatography.

Immunogen: Recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)

Clone: B3

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: Use at 10 μg/mL, WB: Use at 5 μg/mL, Immunohistochemistry: Use at 10 μg/mL, FACS analysis: Use at 10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for CEACAM1

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Alternative Name: CEACAM-1 (CEACAM1 Products)

Background: CEACAM1, BGP, BGP1, BGPI, CD66a,Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1), also known as CD66a and BGP-I, is a 115 kDa type I transmembrane glycoprotein that belongs to the membrane-bound CEA subfamily of the CEA superfamily. On the surface of cells, it forms noncovalent homo- and heterodimers. The extracellular region contains three C2-type Ig-like domains and one N-terminal V-type Ig-like domain. Multiple splice variants involving regions C-terminal to the second C2-type domain (aa 320 and beyond) exist.

Gene ID: 634

NCBI Accession: NM_001205344, NP_001192273

UniProt: P13688