TEK antibody

Catalog No. ABIN1589701

Product Details anti-TEK Antibody

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: TIE-2 antibody

Specificity: Recombinant human soluble extracellular TIE-2

Characteristics: Chromosomal location: 9p21
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-2 as the immunizing antigen.

Purification: Mouse IgG1 antibody (#tek16) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Immunogen: Recombinant human soluble extracellular TIE-2

Clone: Tek16

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 1-2 μg/mL. Western blotting: Use at 2-5 μg/mL. FACS analysis: Use at 2-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

References for anti-TEK antibody (ABIN1589701)

Shahani, Covens, Lavendhomme, Jazouli, Sokal, Peerlinck, Jacquemin: "Human liver sinusoidal endothelial cells but not hepatocytes contain factor VIII." in: Journal of thrombosis and haemostasis : JTH, Vol. 12, Issue 1, pp. 36-42, (2014) (PubMed).

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE-2 (TEK Products)

Background: TEK, TIE2, VMCM, TIE-2, VMCM1, CD202B,Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

Gene ID: 7010

NCBI Accession: NM_000459, NP_000450

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding