Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
Epitopes for Species: H:S157,M:S153,R:S154
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 157 (R-V-SP-N-A).