CD34 antibody

Catalog No. ABIN155167

Product Details anti-CD34 Antibody

Target: CD34

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with CD34 - expressed on lymphoid and myeloid hematopoietic progenitor cells. Also reacts with vascular endothelial cells in normal tissues and in benign and malignant proliferations.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography

Immunogen: Recombinant Human CD34 protein.

Clone: 34C02

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunohistology on Frozen Material.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile Water to a concentration of 1.0 mg/mL.

Buffer: 10 mM PBS, pH 7.4 without preservatives or stabilizers

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody, though stable at RT, is best stored desiccated below 0 °C. Reconstituted antibody is stable at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. CD34, a single chain transmembrane glycoprotein, is selectively expressed on human lymphoid and myeloid hematopoietic progenitor cells.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 947

NCBI Accession: NP_001020280

UniProt: P28906