CD28 antibody (pTyr218)
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- Target See all CD28 Antibodies
- CD28
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Binding Specificity
- AA 171-220, pTyr218
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CD28 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
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CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
PhosphorylationH:Y218 M:Y216
- Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
- Purity
- > 95 %
- Immunogen
- The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
- Isotype
- IgG
- Top Product
- Discover our top product CD28 Primary Antibody
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- Application Notes
- WB: 1:500~1:1000 ELISA: 1:40000
- Comment
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Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Stable at -20°C for at least 1 year.
- Expiry Date
- 12 months
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- Target
- CD28
- Alternative Name
- CD28 (CD28 Products)
- Synonyms
- Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
- Background
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Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen
NCBI Gene Symbol: CD28
- Molecular Weight
- 25 kDa
- Gene ID
- 940
- OMIM
- 186760
- UniProt
- P10747
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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