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Vimentin antibody (pSer50)

Name: Vimentin antibody (pSer50)
SKU: ABIN1449160
Price: 699.6 USD
Availability: InStock

VIM Reactivity: Human, Mouse, Rat WB, IF Host: Rat Monoclonal TM50 unconjugated

Western Blotting (WB) image for anti-Vimentin (VIM) (pSer50) antibody (ABIN1449160) (Vimentin antibody (pSer50))

Immunofluorescence (IF) image for anti-Vimentin (VIM) (pSer50) antibody (ABIN1449160) (Vimentin antibody (pSer50))

2 images

Catalog No. ABIN1449160

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

16

16

15

11

9

9

7

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer50
Reactivity
304

119

116

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
158

130

11

6

5

5

2
Rat
Compatible Secondaries
Clonality
172

144
Monoclonal
Conjugate
197

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
262

115

104

96

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

Sequence

CPSTSRpSLY SSS

Specificity

This antibody reacts with vimentin phosphorylated at Ser50.

Cross-Reactivity (Details)

Species reactivity (tested):Human, mouse, rat

Purification

Protein G agarose

Immunogen

KLH-PV50 peptide

Clone

TM50

Isotype

IgG2a

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VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

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VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative

Without preservative

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Background

Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence.Synonyms: VIM

Gene ID

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis