FAS antibody (Biotin)

Catalog No. ABIN135820

Product Details anti-FAS Antibody (Biotin)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Human/Rat CD95, Mr 45 kDa

Characteristics: Mouse Anti-Human CD95-BIOT

Immunogen: Human CD95 transfected L cells

Clone: DX3

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-PS - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, APC and SPRD conjugates 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

in vivo induction of apoptosis

Restrictions: For Research Use only

Handling

Buffer: 100 tests in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 (FAS Products)

Background: CD95, also known as Fas and Apo-1, is a 40-50 kDa type I transmembrane glycoprotein and a member of the tumor necrosis factor receptor superfamily. It is expressed by activated lymphocytes, monocytes, neutrophils, fibroblasts and cell lines. Fas ligand binding to CD95 induces apoptosis in activated mature lymphocytes thereby playing a role in maintaining peripheral tolerance. Crosslinking of CD95 by the monoclonal antibodies DX2 and DX3 delivers an apoptotic signal to Fas-sensitive cells, indicating that these monoclonal antibodies recognize a functional epitope of CD95.

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity