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CD8 alpha antibody

CD8A Reactivity: Chicken FACS Host: Mouse Monoclonal EP72 unconjugated
Catalog No. ABIN135590
  • Target See all CD8 alpha (CD8A) Antibodies
    CD8 alpha (CD8A) (CD8a Molecule (CD8A))
    Reactivity
    • 180
    • 112
    • 45
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    • 7
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    • 2
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    • 1
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    Chicken
    Host
    • 150
    • 124
    • 96
    Mouse
    Clonality
    • 268
    • 97
    • 2
    Monoclonal
    Conjugate
    • 130
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    • 1
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    This CD8 alpha antibody is un-conjugated
    Application
    • 295
    • 114
    • 84
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    Flow Cytometry (FACS)
    Specificity
    Chicken CD8α, Mr 34 kDa
    Characteristics
    Mouse Anti-Chicken CD8a-UNLB
    Purification
    Purified
    Immunogen
    Chicken splenocytes
    Clone
    EP72
    Isotype
    IgG2b
    Top Product
    Discover our top product CD8A Primary Antibody
  • Application Notes
    • Applications: FC - Quality tested , IHC-FS - Reported in literature
    • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added
    Preservative
    Without preservative
    Handling Advice
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Target
    CD8 alpha (CD8A) (CD8a Molecule (CD8A))
    Alternative Name
    CD8a (CD8A Products)
    Background
    In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain. Chicken CD8 is expressed on approximately 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but < 1 % of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents.
    Pathways
    TCR Signaling
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