CD3 antibody

Catalog No. ABIN135504

Product Details anti-CD3 Antibody

Target: CD3

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Chicken/Pigeon CD3, Mr 20, 19 & 17 kDa

Characteristics: Mouse Anti-Chicken CD3-UNLB

Purification: Purified

Immunogen: Chicken thymocytes and Ig- blood mononuclear cells

Clone: CT-3

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Stim - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE conjugate 0.3 g/106 cells SPRD conjugate 0.2 g/106 cells AF700, AF647, and APC conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro depletion of CD3+ cells, In vivo activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD3 antibody (ABIN135504)

Zhang, Sun, Wang, Chen, Huang, Chen, Chen: "The Postembryonic Development of the Immunological Barrier in the Chicken Spleens." in: Journal of immunology research, Vol. 2019, pp. 6279360, (2019) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Background: CD3 is a member of the T cell receptor-associated CD3 complex.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway